Ecogenomic assessment of Selenium on aquatic ecosystems in a boreal lake

GeoNetwork record: www.gwfnet.net/geonetwork/srv/eng/catalog.search#/metadata/a05adceb-3dc0-4076-b410-83d0103e04b0 Tracking ID under eDNA project: UofS-eDNA-dataset-metadata-7

Data for environmental DNA collection will include water chemistry measurements, water collection, filtration and extraction of DNA/RNA from filters. Metabarcoding data of zooplankton DNA and sedimentary DNA is generated via next-generation sequencing. Bioinformatic pipeline is applied to processing sequence data and convert it to human-readable data.

This project aims to study the ecological effects of Selenium on the aquatic ecosystem by use of environmental DNA (eDNA) metabarcoding. Bacterial, algal, zooplankton and benthic macroinvertebrates communities were characterized by amplicon next-generation sequencing based eDNA metabarcoding. eDNA metabarcoding is being compared with morphology-based species identification for both zooplankton and macroinvertebrate. The data set also serves for the development of tools for risk assessment of eDNA approach. This data set is collected for the project titled Next Generation Solutions to Ensure Healthy Water Resources for Future Generations. This sub-project of eDNA project is collaborated with Se mesocosm study conducted at IISD-ELA. This is a Pillar 3 project under the Global Water Futures Program funded by Canada First Research Excellence Fund.

Freshwater ecosystems are under threat from and being accidental imperiled by mining. The ecosystem responses of freshwater ecosystems to Selenium are also largely unknown due to the extremely high biodiversity. eDNA metabarcoding can be used to monitoring multiple communities in aquatic ecosystems. Controlled mesocosm experiments were conducted at ELA. Collection of eDNA from the water allows for identification of all organisms within a community and is a very useful tool to measure biodiversity. This will allow for predictive measurements of ecosystem health, ensuring that we can maintain healthy water resources for future generations.

Lake 239, IISD - Experimental Lakes Area

data is under embargo.