Thermal Drying Synthesized Density-Tunable and High-Stability Nonthiolated Spherical Nucleic Acids for Split Aptamer and Lateral Flow Biosensors

https://doi.org/10.1016/j.bios.2025.117863

This dataset provides fluorescence assay data demonstrating the tunable DNA density of nonthiolated spherical nucleic acids (SNAs). These nonthiolated SNAs were then employed for oxytetracycline detection using UV-vis spectroscopy and for detecting genetically modified soybean using lateral flow assays (LFAs). Signal acquisition was performed with a Tecan Spark multifunctional microplate reader ( https://www.tecan.com/spark-overview ), with excitation/emission wavelengths set at 467/525 nm for SYBR Green I and 485/520 nm for carboxyfluorescein. Quantitative analysis of the LFA signal intensity data was performed using ImageJ software ( https://fiji.sc/ ).

Wang, X., Liu, J. (2025). Thermal Drying Synthesized Density-Tunable and High-Stability Nonthiolated Spherical Nucleic Acids for Split Aptamer and Lateral Flow Biosensors. Federated Research Data Repository. https://doi.org/10.20383/103.01267

CFI-MSI Project No. 42687 Major Science Initiative Global Water Futures Observatories

Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

https://www.frdr-dfdr.ca/repo/dataset/8f276cdb-f622-4d34-8cba-3a260758959e